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A blood smear, also known as a blood film, is a thin layer of blood spread across a microscope slide and stained in order to view the blood cells microscopically. These are routinely used in histology for the investigation of hematological conditions, such as malarial infection. Proper technique and dexterity is necessary to effectively smear the blood thin and wide enough to visualise the cells.
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Things You'll Need:
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- Appropriate personal protective equipment (PPE): gloves, disposable gown, googles or face shield, mask or respirator
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- Glass microscope slides: preferably with a frosted for labelling
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- Capillary tubes
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- Reagents for specimen preservation: fixatives, stains, etc
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- Pencil or fine-tipped pen with indelible black ink
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Steps:
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1. Review the requested tests to be performed on the specimen.
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2. Obtain a blood sample.
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- Capillary blood is obtained by doing a fingerstick and letting a drop of blood fall from the patient's finger directly to the slide.
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- Venous blood requires venipuncture to obtain. Since it's a more invasive procedure, strict skin asepsis should be performed. Avoid drawing from an existing IV line if possible. Draw blood into EDTA tubes and mix thoroughly to prevent clotting. Label the tubes with at least two patient identifiers.
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